Journal: Endocrinology

Article Title: Deficiency of C1QL1 reduced murine ovarian follicle reserve through intraovarian and endocrine control.

doi: 10.1210/endocr/bqac048

Figure Lengend Snippet: Figure 5. Deficiency of C1QL1 promoted apoptosis and inhibited autophagy in the ovary. (A) Representative images of TUNEL staining of ovarian sections. The green signals indicate representative cells with positive TUNEL staining, which was mainly detected in the large atretic follicles. (B) The intensity of TUNEL-positive signals was shown in charts (n = 4 per group). (C) Representative images of immunofluorescence staining of ovarian sections with LC3B antibody. LC3B-positive signals were found in granulosa cells and oocytes and were weakened in C1QL1-deficient ovaries. (D) The intensity of LC3B-positive signals are shown (n = 4 per group). (E) Apoptosis- and autophagy-related proteins in the ovaries were analyzed by Western blotting. Representative blots are shown in the left panel, and the amount of protein normalized to β-tubulin, which is presented as mean ± SEM, is shown in the right panel. n = 6 per group. *P < 0.05, **P < 0.01, ***P < 0.001. NS, no significance.

Article Snippet: The sections and granulosa cells were stained with anti-rabbit LC3B antibody and Alexa Fluor 594-conjugated secondary antibody and mounted with DAPI (Boster).

Techniques: TUNEL Assay, Staining, Immunofluorescence, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Effect of Autophagy Regulated by Sirt1/FoxO1 Pathway on the Release of Factors Promoting Thrombosis from Vascular Endothelial Cells

doi: 10.3390/ijms20174132

Figure Lengend Snippet: ( A ) Viability of HUVEC treated with different concentrations of ox-LDL. ( B ) Concentration of von Willebrand factor (vWF) in media. ( C ) Expression of vWF on cytomembrane. ( D ) Expression of P-selectin on cytomembrane. ( E ) Expression of Sirt1, FoxO1, LC3-II/I and p62. Values are presented as the means ± SD (MTT and ELISA assay: n = 6, flow cytometry and Western Blot: n = 3). Compared with control groups at each time point, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Rabbit anti-mouse antibodies and rabbit anti-human LC3, p62 and β-actin antibodies were obtained from Wanleibio (Shenyang, China).

Techniques: Concentration Assay, Expressing, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: Effect of Autophagy Regulated by Sirt1/FoxO1 Pathway on the Release of Factors Promoting Thrombosis from Vascular Endothelial Cells

doi: 10.3390/ijms20174132

Figure Lengend Snippet: The viability of HUVEC treated with RSV ( A ) or SRT1720 ( B ). ( C ) Expression of Sirt1, FoxO1, Ac-FoxO1, LC3-II/I and p62 in HUVEC treated with RSV. ( D ) Expression of Sirt1, FoxO1, Ac-FoxO1, LC3-II/I and p62 in HUVEC treated with SRT1720. Values are presented as the means ± SD (MTT assay: n = 6; Western Blot: n = 3). Compared with the ox-LDL group, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Rabbit anti-mouse antibodies and rabbit anti-human LC3, p62 and β-actin antibodies were obtained from Wanleibio (Shenyang, China).

Techniques: Expressing, MTT Assay, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Effect of Autophagy Regulated by Sirt1/FoxO1 Pathway on the Release of Factors Promoting Thrombosis from Vascular Endothelial Cells

doi: 10.3390/ijms20174132

Figure Lengend Snippet: Effect of gene silencing Rab7 on the expression of Rab7, LC3-II/I and p62. HUVECs were transfected with the Rab7 siRNA for 24 h incubated with 100 μg/mL ox-LDL and 10 μM RSV or 5 μM SRT1720 for 12 h. Expression of Rab7, LC3-II/I and p62 in HUVECs treated with RSV or SRT1720 was determined by Western blot. Values are presented as the means ± SD ( n = 3). Statistical significance between the ox-LDL treated group and other groups was determined by one-way ANOVA: Compared with the ox-LDL group, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Rabbit anti-mouse antibodies and rabbit anti-human LC3, p62 and β-actin antibodies were obtained from Wanleibio (Shenyang, China).

Techniques: Expressing, Transfection, Incubation, Western Blot

Journal: International Journal of Biological Sciences

Article Title: Slit2/Robo1 Mitigates DSS-induced Ulcerative Colitis by Activating Autophagy in Intestinal Stem Cell

doi: 10.7150/ijbs.42331

Figure Lengend Snippet: Expression of the autophagy proteins in DSS-induced mice. The coexpression of Lgr5 + colonic stem cells and the autophagy protein LC3 in (A) WT Slit and Slit2-Tg mice and (B) WT Robo1/2 and Robo1/2 +/- mice. Lgr5 (green) and LC3 (red) expression in the colonic epithelium. Sections were counterstained with DAPI (blue). (n=5 in each group; scale bar = 50 μm); (C) the photograph of the crypt isolated from colon tissue (scale bar= 200μm); (D) protein expression of LC3II/I in (D) WT Slit and Slit2-Tg mice and (F) WT Robo1/2 and Robo1/2 +/- mice (n=4); The expression of P62 in (E) WT Slit and Slit2-Tg mice and (G) WT Robo1/2 and Robo1/2 +/- mice (n=3-4 in each group). Detection of β-actin served as a loading control. Quantification of bands is expressed as density ratio of indicated protein/β-actin (A.U.); data are present as means ± SEM. *P<0.05, **P<0.01, ***P<0.001.

Article Snippet: Rabbit anti-mouse LC3 polyclonal antibody (1:500, ABclonal), rabbit anti-mouse p62 polyclonal antibody (1:500, Affinity Biosciences), and mouse anti-mouse β-actin (1:10000, Boster) were added and incubated at 4°C overnight.

Techniques: Expressing, Isolation, Control